Mentor/s

Professor Mark Jareb

Location

University Commons

Start Day/Time

4-21-2017 1:00 PM

End Day/Time

4-21-2017 3:00 PM

Abstract

The correct targeting of proteins to axons and dendrites of neurons is essential for the proper development of the nervous system. L1CAM is an axonally-targeted protein responsible for multiple aspects of neuronal development. L1CAM mutations are known to result in a developmental syndrome characterized by cognitive and motor disabilities. We investigated the cellular distribution of known L1CAM mutant proteins, P941L and D544N, in cultured embryonic chick forebrain neurons to test the hypothesis that aberrant protein targeting of these mutants plays a role in the developmental abnormalities associated with the syndrome. Preliminary data suggests that the P941L L1CAM mutant is targeted normally to the axon suggesting that downstream signaling events are abnormal. In contrast, the D544N L1CAM mutant does not appear to reach the cell surface of the neuron.

College

College of Arts and Sciences

College and Major available

Biology

Keywords

Nervous system, Neurons, Proteins, Mutations

Document Type

Poster

Creative Commons License

Creative Commons Attribution-Noncommercial-Share Alike 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 License.

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Apr 21st, 1:00 PM Apr 21st, 3:00 PM

Protein Targeting of L1CAM Mutants in Cultured Neurons

University Commons

The correct targeting of proteins to axons and dendrites of neurons is essential for the proper development of the nervous system. L1CAM is an axonally-targeted protein responsible for multiple aspects of neuronal development. L1CAM mutations are known to result in a developmental syndrome characterized by cognitive and motor disabilities. We investigated the cellular distribution of known L1CAM mutant proteins, P941L and D544N, in cultured embryonic chick forebrain neurons to test the hypothesis that aberrant protein targeting of these mutants plays a role in the developmental abnormalities associated with the syndrome. Preliminary data suggests that the P941L L1CAM mutant is targeted normally to the axon suggesting that downstream signaling events are abnormal. In contrast, the D544N L1CAM mutant does not appear to reach the cell surface of the neuron.

 

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