Document Type
Article
Publication Date
10-2016
Abstract
The mixed microbial communities that occur naturally on lignocellulosic feedstocks can provide feedstock-specific enzyme mixtures to saccharify lignocelluloses. Bacterial-fungal communities were enriched from switchgrass bales to deconstruct ammonia-pretreated switchgrass (DSG). Correlation analysis was carried out to elucidate the relationship between microbial decomposition of DSG by these communities, enzymatic activities produced and enzymatic saccharification of DSG using these enzyme mixtures. Results of the analysis showed that β glucosidase activities and xylosidase activities limited the extent of microbial deconstruction and enzymatic saccharification of DSG. The results also underlined the importance of ligninase activity for the enzymatic saccharification of pretreated lignocellulosic feedstock. The bacterial fungal communities developed in this research can be used to produce enzyme mixtures to deconstruct DSG, and the results from the correlation analysis can be used to optimize these enzyme mixtures for efficient saccharification of DSG to produce second-generation biofuels.
DOI
10.1016/j.biortech.2016.07.041
Recommended Citation
Jain, Abhiney; Pelle, H. Sandra; and Henson, J. Michael, "Correlation Analysis of Enzyme activities and Deconstruction of Ammonia-pretreated Switchgrass by Bacterial-fungal Communities" (2016). Biology Faculty Publications. 115.
https://digitalcommons.sacredheart.edu/bio_fac/115
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Comments
PMID: 27469088
Version posted is the authors' accepted manuscript.
Published in its final edited form as:
Jain, A., Bediako, S.H., Henson, J.M. (2016). Correlation analysis of enzyme activities and deconstruction of ammonia-pretreated switchgrass by bacterial-fungal communities. Bioresource Technology, 218, 1082–1089. doi: 10.1016/j.biortech.2016.07.041