Participation Type

Poster

Mentor/s

Dr. Suzanne Deschenes

College

College of Arts and Sciences

Location

University Commons

Start Day/Time

4-24-2019 2:00 PM

End Day/Time

4-24-2019 5:00 PM

Abstract

In 2015, the CDC reported that the number of obese adults in the U.S. was 93.3 million. A well-known association between metabolic disorders such as diabetes and obesity exists. Diabetes leads to increased levels of blood glucose due to a decrease in insulin production or insulin resistance. Rather than being used as energy within cells, excess blood glucose is stored in adipose tissue, leading to obesity. The current research literature shows that capsaicin, a constituent of chili peppers, increases glucose uptake in C2C12 mouse skeletal myoblasts, making capsaicin a potential therapeutic for obesity and diabetes.Capsaicin activates the AMP- activated protein kinase (AMPK) signal transduction pathway through binding with the endocannabinoid receptor CB1. AMPK phosphorylates Acetyl-coA Carboxylase (ACC) causing ACC to then become inactive, resulting in increased glucose uptake and decreased lipid synthesis. We would like to explore the potential of other CB1 agonists to stimulate glucose uptake. Differentiated C2C12 cells will be exposed to 25 uM anandamide, a CB1-like agonist, for30 or 60 minutes along with a solvent control and the positive control of 100μM capsaicin. Phosphorylation of ACC will be detected via western blot and glucose uptake assays will be performed to assess the effectiveness of anandamide in stimulating differentiated C2C12 cells to respond to elevated glucose. Data will be presented and compared to related studies in the literature.

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Apr 24th, 2:00 PM Apr 24th, 5:00 PM

The Impact of Anadamide on the AMPK Pathyway of C2C12 Cells

University Commons

In 2015, the CDC reported that the number of obese adults in the U.S. was 93.3 million. A well-known association between metabolic disorders such as diabetes and obesity exists. Diabetes leads to increased levels of blood glucose due to a decrease in insulin production or insulin resistance. Rather than being used as energy within cells, excess blood glucose is stored in adipose tissue, leading to obesity. The current research literature shows that capsaicin, a constituent of chili peppers, increases glucose uptake in C2C12 mouse skeletal myoblasts, making capsaicin a potential therapeutic for obesity and diabetes.Capsaicin activates the AMP- activated protein kinase (AMPK) signal transduction pathway through binding with the endocannabinoid receptor CB1. AMPK phosphorylates Acetyl-coA Carboxylase (ACC) causing ACC to then become inactive, resulting in increased glucose uptake and decreased lipid synthesis. We would like to explore the potential of other CB1 agonists to stimulate glucose uptake. Differentiated C2C12 cells will be exposed to 25 uM anandamide, a CB1-like agonist, for30 or 60 minutes along with a solvent control and the positive control of 100μM capsaicin. Phosphorylation of ACC will be detected via western blot and glucose uptake assays will be performed to assess the effectiveness of anandamide in stimulating differentiated C2C12 cells to respond to elevated glucose. Data will be presented and compared to related studies in the literature.