Title of Poster or Paper

Investigation of the Role of Deregulated microRNAs in Melanoma Pathogenesis and progress

Mentor/s

Sankhiros Babapoor

Participation Type

Poster

Abstract

The incidence of melanoma has continually increased mortality rates over the past decade in the United States. In 2013, it was estimated that 76,690 individuals (both male and female) were diagnosed with new cases of cutaneous melanoma and out of those cases, 9,480 resulted in death. MicroRNA(miRNA)s are endogenous, 22 nucleotide non-coding small RNAs, which can regulate gene expression in animals and plants by complementary base-pairing to the mRNAs of target genes- which specifies mRNA cleavage or translation repression. We have established a distinct set of miRNAs associated with invasive and aggressive melanoma phenotype. MiR-21, miR-30b, miR-146a, miR-204 and miR-4454, and investigated the role of them in the invasion and migration of a malignant melanoma cell lines (A375P and Sk-Mel-23). The results indicated that miR-30b significantly decreased (P-value= 0.01) the migration rate of A-375P 48h post scratch. An opposite outcome was seen after transfecting A-375P and Sk-Mel-26 cells with miR-4454 and the migration rate significantly increased (P-value= 0.01 and P-value= 0.018 accordingly) after 48h post scratch. This study indicated that while the miR-30b act as a tumor suppressor, the miR-4454 act as oncomiR .

College and Major available

Biology

Location

Digital Commons & West Campus West Building

Start Day/Time

4-29-2022 1:00 PM

End Day/Time

4-29-2022 4:00 PM

Students' Information

Jamie Maresca, Biology Major, May 2022

Maximillian Lilo, Biology Major, May 2022

Simona Bruno, Biology Major, May 2022

Noelle Butera, Molecular and Cellular Biology Major, December 2022

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Apr 29th, 1:00 PM Apr 29th, 4:00 PM

Investigation of the Role of Deregulated microRNAs in Melanoma Pathogenesis and progress

Digital Commons & West Campus West Building

The incidence of melanoma has continually increased mortality rates over the past decade in the United States. In 2013, it was estimated that 76,690 individuals (both male and female) were diagnosed with new cases of cutaneous melanoma and out of those cases, 9,480 resulted in death. MicroRNA(miRNA)s are endogenous, 22 nucleotide non-coding small RNAs, which can regulate gene expression in animals and plants by complementary base-pairing to the mRNAs of target genes- which specifies mRNA cleavage or translation repression. We have established a distinct set of miRNAs associated with invasive and aggressive melanoma phenotype. MiR-21, miR-30b, miR-146a, miR-204 and miR-4454, and investigated the role of them in the invasion and migration of a malignant melanoma cell lines (A375P and Sk-Mel-23). The results indicated that miR-30b significantly decreased (P-value= 0.01) the migration rate of A-375P 48h post scratch. An opposite outcome was seen after transfecting A-375P and Sk-Mel-26 cells with miR-4454 and the migration rate significantly increased (P-value= 0.01 and P-value= 0.018 accordingly) after 48h post scratch. This study indicated that while the miR-30b act as a tumor suppressor, the miR-4454 act as oncomiR .