Butyl Benzyl Phthalate (BBP) Disrupts Neuromast Development in Embryonic Zebrafish

Document Type

Peer-Reviewed Article

Publication Date



Butyl benzyl phthalate (BBP) is found in common household and industrial products world-wide. Phthalates are not covalently bound to plastics and continuously leach into the soil, sediment and aquatic environments. The lateral line system of fish is a mechanosensory system composed of neuromasts essential for survival behaviors including rheotaxis, schooling and predator avoidance. Here, we investigated the developmental toxicity of BBP on the developing lateral line neuromasts in zebrafish. Embryos were treated at gastrula stage with BBP and analyzed by DASPEI staining at 4 days post fertilization. We find that BBP negatively affects neuromast development leading to loss of DASPEI signal in neuromasts in a concentration dependent manner.


Online ahead of print February 15, 2024

At the time of publication Evelyn Paquette, John Paul Giacalone, and Michael Fumo were undergraduate students at sacred Heart University