Spectrophotometric Detrmination of Nitrite with Acidified Solution of Indole

Date of Award


Degree Type


Degree Name

Master of Science (MS)



First Advisor

Dhia A. Habboush, Ph.D.


The determination of nitrite ions is important because they are considered harmful to human health in high concentrations. There are many of instruments and techniques that are used to determine nitrite. This study sought to explore the determination of nitrite concentration using UV visible spectroscopy. Reduced chemical hazard and simplicity represent improvement over existing methods. It is found that nitrite determination using UV visible spectrophotometric methods is mostly done using a single reagent which is indole reagent in an acidic media. Indole is used as an indicator and in presence of nitrite the color of the solution changes to pink red. Detection limit for the method is less than 1 ppm of Nitrite.


Master's thesis submitted to the faculty of Sacred Heart University's Chemistry Program in partial fulfillment of the requirements for the degree of Master of Chemistry.

The original title contains the spelling error.