Mentor/s
Professor Mark Jareb
Participation Type
Poster
Abstract
The correct targeting of proteins to axons and dendrites of neurons is essential for the proper development of the nervous system. L1CAM is an axonally-targeted protein responsible for multiple aspects of neuronal development. L1CAM mutations are known to result in a developmental syndrome characterized by cognitive and motor disabilities. We investigated the cellular distribution of known L1CAM mutant proteins, P941L and D544N, in cultured embryonic chick forebrain neurons to test the hypothesis that aberrant protein targeting of these mutants plays a role in the developmental abnormalities associated with the syndrome. Preliminary data suggests that the P941L L1CAM mutant is targeted normally to the axon suggesting that downstream signaling events are abnormal. In contrast, the D544N L1CAM mutant does not appear to reach the cell surface of the neuron.
College and Major available
Biology
Location
University Commons
Start Day/Time
4-21-2017 1:00 PM
End Day/Time
4-21-2017 3:00 PM
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 License.
Protein Targeting of L1CAM Mutants in Cultured Neurons
University Commons
The correct targeting of proteins to axons and dendrites of neurons is essential for the proper development of the nervous system. L1CAM is an axonally-targeted protein responsible for multiple aspects of neuronal development. L1CAM mutations are known to result in a developmental syndrome characterized by cognitive and motor disabilities. We investigated the cellular distribution of known L1CAM mutant proteins, P941L and D544N, in cultured embryonic chick forebrain neurons to test the hypothesis that aberrant protein targeting of these mutants plays a role in the developmental abnormalities associated with the syndrome. Preliminary data suggests that the P941L L1CAM mutant is targeted normally to the axon suggesting that downstream signaling events are abnormal. In contrast, the D544N L1CAM mutant does not appear to reach the cell surface of the neuron.
