Mentor/s
Dr. Eun Yong Shim, Dr. Sang Lee
Participation Type
Poster
Abstract
The proliferation of cancer strongly depends on flawless cell replication. DNA repair mechanisms are responsible for the cells to replicate without errors. We focused on studying apex2 and its function in the DNA repair pathway of mammalian cells. In BRCA-mutant tumors, apex2 has been identified as a potential synthetic lethal target. Studies in yeast showed that apn2 reduces the frequency of mutations and repairs damage caused by top1. We used CRISPR-Cas9 to knockout apex2 gene in HCT116 and U-2OS and tested genetic interaction between apex2, tdp1, ercc4 and apex1 using siRNA and drug sensitivity. Our results suggest that knockout of apex2 together with knockdown of tdp1 and ercc4 increased drug sensitivity.
College and Major available
Biology
Location
Digital Commons
Start Day/Time
5-5-2021 1:00 PM
End Day/Time
5-5-2021 4:00 PM
Comments
Video Recording of Presentation: https://sacredheart-edu.zoom.us/rec/share/-41fJ6bitOtGQQ3pfTn-tK-at9e8U8H20XWvRkJVjHoKxckZugT9hY8hcE7T8SUU.RrQcuzL1f6KnrG4r?startTime=1619644759000
Prize Categories
Best Multidisciplinary Research or Collaboration, Most Scholarly Impact or Potential, Most Creative
Role of APEX2 in DNA Repair Mechanisms
Digital Commons
The proliferation of cancer strongly depends on flawless cell replication. DNA repair mechanisms are responsible for the cells to replicate without errors. We focused on studying apex2 and its function in the DNA repair pathway of mammalian cells. In BRCA-mutant tumors, apex2 has been identified as a potential synthetic lethal target. Studies in yeast showed that apn2 reduces the frequency of mutations and repairs damage caused by top1. We used CRISPR-Cas9 to knockout apex2 gene in HCT116 and U-2OS and tested genetic interaction between apex2, tdp1, ercc4 and apex1 using siRNA and drug sensitivity. Our results suggest that knockout of apex2 together with knockdown of tdp1 and ercc4 increased drug sensitivity.
Students' Information
Mirjam Grebenc, Molecular and Cellular Biology, Honors, 2021
Please use the Link to Full Text for the correct Recording of this presentation. Technical difficulties are preventing the removal of the Audio recording in the middle of the page.