First and Last Name/s of Presenters

Mirjam GrebencFollow

Title of Poster or Paper

Role of APEX2 in DNA Repair Mechanisms

Mentor/s

Dr. Eun Yong Shim, Dr. Sang Lee

Participation Type

Poster

Abstract

The proliferation of cancer strongly depends on flawless cell replication. DNA repair mechanisms are responsible for the cells to replicate without errors. We focused on studying apex2 and its function in the DNA repair pathway of mammalian cells. In BRCA-mutant tumors, apex2 has been identified as a potential synthetic lethal target. Studies in yeast showed that apn2 reduces the frequency of mutations and repairs damage caused by top1. We used CRISPR-Cas9 to knockout apex2 gene in HCT116 and U-2OS and tested genetic interaction between apex2, tdp1, ercc4 and apex1 using siRNA and drug sensitivity. Our results suggest that knockout of apex2 together with knockdown of tdp1 and ercc4 increased drug sensitivity.

College and Major available

Biology

Course Name and Number, Professor Name

INTERNSHIP-BIOLOGY MAJORS BI-360-IG, Nicole Roy

Location

Digital Commons

Start Day/Time

5-5-2021 1:00 PM

End Day/Time

5-5-2021 4:00 PM

Students' Information

Mirjam Grebenc, Molecular and Cellular Biology, Honors, 2021

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Comments

Video Recording of Presentation: https://sacredheart-edu.zoom.us/rec/share/-41fJ6bitOtGQQ3pfTn-tK-at9e8U8H20XWvRkJVjHoKxckZugT9hY8hcE7T8SUU.RrQcuzL1f6KnrG4r?startTime=1619644759000

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May 5th, 1:00 PM May 5th, 4:00 PM

Role of APEX2 in DNA Repair Mechanisms

Digital Commons

The proliferation of cancer strongly depends on flawless cell replication. DNA repair mechanisms are responsible for the cells to replicate without errors. We focused on studying apex2 and its function in the DNA repair pathway of mammalian cells. In BRCA-mutant tumors, apex2 has been identified as a potential synthetic lethal target. Studies in yeast showed that apn2 reduces the frequency of mutations and repairs damage caused by top1. We used CRISPR-Cas9 to knockout apex2 gene in HCT116 and U-2OS and tested genetic interaction between apex2, tdp1, ercc4 and apex1 using siRNA and drug sensitivity. Our results suggest that knockout of apex2 together with knockdown of tdp1 and ercc4 increased drug sensitivity.

 

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