Mentor/s
Dr. Bartholomew Dr. Woronik Academic advisor: Dr. Stoehr
Participation Type
Paper Talk
Abstract
Species within a converging or shared gene regulatory network (GRN) can have the instance where a gene may be gained and lost over time due to the evolution of that GRN. As an example of this, one gene of interest that seems to affect tail tip morphogenesis (TTM) among nematodes is dmd-3. In one of the species of nematodes, C. elegans, dmd-3 was found to be required and sufficient for TTM. Knowing this, a hypothesis was made that dmd-3 is a repeatedly co-opted gene (master regulator), also known as an evolutionary hotspot, where it would be a gene that was required and sufficient to bring about TTM among all nematodes that have the ability to display TTM – this is the hotspot hypothesis. Our results from the experiment did not support the hotspot hypothesis, where even though an O. tipulae sample contained the mutation to yield dmd-3 (-), TTM still occurred. Taking these results into account, it can be concluded that dmd-3 did not play a role in TTM and is not conserved from evolution in the GRN. This tells us how the evolution of the GRN could potentially occur through mutations in cis-regulatory elements (CREs), co-option of transposable elements (TEs) as new CREs, loss of transcriptional inputs in existing CREs, and/or remodeling of CREs (Carroll 2008).
College and Major available
Biology
Location
Session 8: Digital Commons & West Campus West Building Room 140
Start Day/Time
4-26-2024 9:00 AM
End Day/Time
4-26-2024 10:15 AM
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License
Hotspot hypothesis on Oscheius tipulae using CRISPR/Cas9
Session 8: Digital Commons & West Campus West Building Room 140
Species within a converging or shared gene regulatory network (GRN) can have the instance where a gene may be gained and lost over time due to the evolution of that GRN. As an example of this, one gene of interest that seems to affect tail tip morphogenesis (TTM) among nematodes is dmd-3. In one of the species of nematodes, C. elegans, dmd-3 was found to be required and sufficient for TTM. Knowing this, a hypothesis was made that dmd-3 is a repeatedly co-opted gene (master regulator), also known as an evolutionary hotspot, where it would be a gene that was required and sufficient to bring about TTM among all nematodes that have the ability to display TTM – this is the hotspot hypothesis. Our results from the experiment did not support the hotspot hypothesis, where even though an O. tipulae sample contained the mutation to yield dmd-3 (-), TTM still occurred. Taking these results into account, it can be concluded that dmd-3 did not play a role in TTM and is not conserved from evolution in the GRN. This tells us how the evolution of the GRN could potentially occur through mutations in cis-regulatory elements (CREs), co-option of transposable elements (TEs) as new CREs, loss of transcriptional inputs in existing CREs, and/or remodeling of CREs (Carroll 2008).
Students' Information
Name: Rebecca Margolnick-Fernandez
Major: Biology
Honors Student: Yes
Year of Graduation: 2024