Mentor/s
Mark Jareb
Participation Type
Poster
Abstract
The amyloid precursor protein (APP) has commonly shown to lead to the pathogenesis of Alzheimer’s disease through its cleavage into amyloid-β plaques that aggregate in the brain, causing neurodegeneration. To better understand the pathophysiology of Alzheimer's disease, researching the normal targeting and processing of APP in neurons can reveal many of the processes that lead to the aggregation of senile plaques in the brain. We have expressed a FLAG-tagged human APP construct in cultured embryonic chick forebrain neurons and visualized its distribution using immunocytochemistry and fluorescent microscopy. Initial findings show that APP is expressed in cell body, dendrites, and axons of cultured chick neurons.
College and Major available
Biology, Neuroscience
Location
Digital Commons & West Campus West Building University Commons
Start Day/Time
4-26-2024 12:00 PM
End Day/Time
4-2024 2:00 PM
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License
Prize Categories
Most Scholarly Impact or Potential, Best Visuals, Best Writing
Protein Targeting of Amyloid Precursor Protein in Cultured Chick Neurons
Digital Commons & West Campus West Building University Commons
The amyloid precursor protein (APP) has commonly shown to lead to the pathogenesis of Alzheimer’s disease through its cleavage into amyloid-β plaques that aggregate in the brain, causing neurodegeneration. To better understand the pathophysiology of Alzheimer's disease, researching the normal targeting and processing of APP in neurons can reveal many of the processes that lead to the aggregation of senile plaques in the brain. We have expressed a FLAG-tagged human APP construct in cultured embryonic chick forebrain neurons and visualized its distribution using immunocytochemistry and fluorescent microscopy. Initial findings show that APP is expressed in cell body, dendrites, and axons of cultured chick neurons.
Students' Information
Isabella Pace, Biology, Honors, 2024
Joseph Tenedine, Neuroscience, 2025